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filexlib. Abstract. Cerebrospinal fluid (CSF) cytology, i.e., the cytologic evaluation of its cellular composition, forms an integral part of the neurologist's armamentarium. Total and differential cell counts provide important first information across a spectrum of pathologic conditions involving the central nervous system and its coverings.
Cytospin Protocol. Wash 105 cells in cold 2% FCS-PBS twice and dilute in 100 (l of cold 1% BSA-PBS. Be sure to keep all samples on ice. Place slides and filters into appropriate slots in the cytospin with the cardboard filters facing the center of the cytospin. In the event that there are few cells available, aliquot about 100 (l of cold 1% BSA Shandon Cytospin 4 Service Manual . Description The Sealed Head Cytoclip Slide Clip Inner Bowl Lid Assembly Locking Device Kit Ball Moulded Lid Sealed Head Hub Sealed Head Seal Kit (Each Sealed Head requires 3 different seals - includes 1 of each type of seal required) Cone Seal Lid Seal O-Ring Seal Support Plate Thumb Nut Part number
Table 2. Cytocentrifugation parameters of the Cytospin 3 in the work-up of BAL fluid. Cells/ml number of drops Acceleration Speed Time < 50.000 7 Low 650 rpm 20 min 50.000 - 100.000 5 - 4 Low 650 rpm 10 min 10.000 - 200.000 4 - 3 Low 650 rpm 10 min 200.000 - 300.000 3 Low 650 rpm 10 min 300.000 - 400.000 3 - 2 Low 650 rpm
This manual is for use only by the individuals to whom it has been made available by 'Thermo Electron Corporatién. Contact Addresses Clinical Diagnostics Clinical Diagnostics Anatomical Pathology Anatomical Pathology 93-96 Chadwick Road 171 Industry Drive Astmoor, Runcorn, Pittsburgh Cheshire, WA7 1PR, UK PA.15275, USA Tel: +44 (0) 1928
Cytospin Protocol (Greg A. Perry, Ph.D.) Equipment: Cell preparation Microscope slides Cytocentrifuge (Shandon) Cytospin slide chambers & blotters Reagents: 3. Add 200ul of each cell suspension to a slide chamber. 4. Spin at 800 rpm for 5 minutes. 5. Carefully remove slide(s) from cytocentrifuge and allow to air dry prior to staining.
Cytospin technique is a method of performing thin-layer preparation from fluids through a cytocentrifugation process. Principle During the procedure, the material is centrifuged and the cells are deposited onto a vertical microscope slide. This procedure permits a cell separation from the fluid medium. Methodology
To prepare cytocentrifuge smears, a funnel assembly is attached to the front of a microscope slide. The surface of the funnel assembly that is in contact with the slide is lined with filter paper to absorb excess fluid. A few drops of fluid are placed in the funnel.
The cytospin deposits thin layer preparations of suspension cells onto slides. Load the cells and set it running. The cell density, loading volume, and spin speed should be optimized for each cell type. For example, larger cells require a slower speed.
Close the Cytospin lid and centrifuge at 1000 × g for 5 min. Remove the slides, filter cards, and cytofunnels from the centrifuge. Be careful not to move the filter paper on the slide and so damage/smudge the cell preparation. If desired, then the glass slides and filter cards can be removed from the cytofunnel, turned upside down before being
Cytospin (CPT 88108)* (CPT 88162)† Specimen preparation 15 13.5 13.5 Screening time 5 5 8 (20, if CPS) CPS: concentrated pull smears. * Includes 2 fixed cytospins. † Includes 2 fixed cytospins and 1 air-dried cytospin. 146 CANCER (CANCER CYTOPATHOLOGY) June 25, 2006 / Volume 108 / Number 3
Cytospin (CPT 88108)* (CPT 88162)† Specimen preparation 15 13.5 13.5 Screening time 5 5 8 (20, if CPS) CPS: concentrated pull smears. * Includes 2 fixed cytospins. † Includes 2 fixed cytospins and 1 air-dried cytospin. 146 CANCER (CANCER CYTOPATHOLOGY) June 25, 2006 / Volume 108 / Number 3
Thermo Fisher Scientific
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